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View Project Dingledine-5R01NS031373-10
Project Summary
Status: Public  
Publications: 1 Published
 		 
Project Detail Data Detail
Platform: Affymetrix MIAME Areas Compliance
Species: Mouse Array Design Detail true
Organ/Tissue Type: Experiment Detail false
Organ Region: hippocampus, left Sample Detail false
Cell Type: Hybridization Detail false
Study Type: subclassification Measurement Detail false
Disease/Condition:
Replicates: 0
Expected Samples:  
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Available Actions
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Investigator Contact Detail
Name Ray Dingledine
Street Address: department of pharmacology
Emory University School of Medicine, 1510 Clifton Rd
City, State/Province: Atlanta , GA
Zip/Postal Code: 30322
Country: United States
Work Phone: 4047275982
Fax: 4047270365
E-mail: rdingledine@pharm.emory.edu
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Proposal Detail
Grant: 5R01NS031373-10
Status: Public
Service Type: Start to Finish Profiling
IACUC: 066-2001
IACUC date: 2001-05-24
Study Relevance:
Epilepsy affects between 1-2% of people worldwide and is defined symptomatically by the appearance of spontaneous, recurrent seizures. The ketogenic diet (KD) is an anticonvulsant treatment that has been used to manage medically-intractable epilepsies. Both clinical and experimental reports show that the KD can be used to treat a variety of seizure types. During KD treatment, ketone bodies, rather than glucose, become the major energy substrate for the brain; somehow, metabolic conversion confers a potent anticonvulsant effect. The KD requires 10-12 days to become maximally effective, suggesting that changes in gene expression are involved in its anticonvulsant action. Despite its use for over 80 years, the mechanism of the diet remains unknown. It is expected that this work will help elucidate the underlying anticonvulsant action of the KD and contribute to future, rationale-based drug development.
Hypothesis:
We hypothesize that gene expression patterns induced by alterations in metabolic pathways are critically involved with the anticonvulsant effects of the KD in mice.
Specific Aim:
We propose to identify sets of genes activated/repressed within hippocampus after treatment with a KD.
Experimental Procedure and Design:
Mice (n=44, 129svJ) will be used for these experiments. Animals will be fed either a KD (n=22) or control diet (n=22) for 14 days. After this time, ten animals from each group will be subjected to flurothyl seizure testing to confirm seizure threshold elevation. Of the remaining KD- (n=12) or control-fed (n=12) animals, two left hippocampi will be pooled together such that six arrays from each treatment group can be tested. This is expected to reduce biological variability. Right hippocampi (n=12/group) will be maintained in our lab for corroborative analysis via RT-PCR and immunohistochemistry. All tissue samples will be rapidly dissected, frozen in liquid nitrogen, and stored at -80C until shipping/corroborative testing. Data analysis will be completed with Significance of Array Measurement (SAM) program using ANOVA from protection for multiple comparisons. Please use the Affy chip with known genes in lieu of the chip containing mostly EST's.
Experimental Factors:
Conditions that are tested in the experiment. At least one is required. Experimental factors are the independent variables in the experiment.
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Experimental Factors is empty.
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Project Samples  This section lists the samples that are associated with this project. Individual sample details can be viewed by clicking on the View Sample icon to the right of the sample. If samples are selectable for analysis or for addition to a virtual
Samples associated with this project.
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Action Button Key
View Sample View Sample  
Name Description Extracts  
Ketogenic 1-2 Hippocampi, left 1
Ketogenic 3-4 Hippocampi, left 1
Ketogenic 5-6 Hippocampi, left 1
Ketogenic 7-8 Hippocampi, left 1
Ketogenic 9-10 Hippocampi, left 1
Ketogenic 11-12 Hippocampi, left 0
Control 1-2 Hippocampi, left 1
Control 3-4 Hippocampi, left 1
Control 5-6 Hippocampi, left 1
Control 7-8 Hippocampi, left 1
Control 9-10 Hippocampi, left 1
Control 11-12 Hippocampi, left 1
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Project Hybridizations 

Action Button Key
View Hybridization View Hybridization  
Name Array Labeled Extract Hybridization Protocol  
RAE230A_1_hyb RAE230A_1 Ketogenic 1-2_e1_le1
RAE230A_2_hyb RAE230A_2 Ketogenic 3-4_e1_le1
RAE230A_3_hyb RAE230A_3 Ketogenic 5-6_e1_le1
RAE230A_4_hyb RAE230A_4 Ketogenic 7-8_e1_le1
RAE230A_5_hyb RAE230A_5 Ketogenic 9-10_e1_le1
RAE230A_6_hyb RAE230A_6 Control 1-2_e1_le1
RAE230A_7_hyb RAE230A_7 Control 3-4_e1_le1
RAE230A_8_hyb RAE230A_8 Control 5-6_e1_le1
RAE230A_9_hyb RAE230A_9 Control 7-8_e1_le1
RAE230A_10_hyb RAE230A_10 Control 9-10_e1_le1
RAE230A_11_hyb RAE230A_11 Control 11-12_e1_le1
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Project Reference Files
File Name Size
Dingledine Signal and P Calls for 48 chips GOOD.xls 26.30 Mb
NINDS-CON-CA3-10a-RAE.CEL 3.45 Mb
NINDS-CON-CA3-10a-RAE.CHP 3.43 Mb
NINDS-CON-CA3-13a-RAE.CEL 3.45 Mb
NINDS-CON-CA3-13a-RAE.CHP 3.43 Mb
NINDS-CON-CA3-19a-RAE.CEL 3.45 Mb
NINDS-CON-CA3-19a-RAE.CHP 3.43 Mb
NINDS-CON-CA3-22a-RAE.CEL 3.45 Mb
NINDS-CON-CA3-22a-RAE.CHP 3.43 Mb
NINDS-CON-CA3-26a-RAE.CEL 3.45 Mb
NINDS-CON-CA3-26a-RAE.CHP 3.43 Mb
NINDS-CON-CA3-3a-RAE.CEL 3.45 Mb
NINDS-CON-CA3-3a-RAE.CHP 3.43 Mb
NINDS-CON-CA3-A5a-RAE.CEL 3.45 Mb
NINDS-CON-CA3-A5a-RAE.CHP 3.43 Mb
NINDS-CON-CA3-A8a-RAE.CEL 8.44 Mb
NINDS-CON-CA3-A8a-RAE.CHP 203.0 Kb
NINDS-CON-DG-10a-RAE.CEL 3.45 Mb
NINDS-CON-DG-10a-RAE.CHP 3.43 Mb
NINDS-CON-DG-13a-RAE.CEL 3.45 Mb
NINDS-CON-DG-13a-RAE.CHP 3.43 Mb
NINDS-CON-DG-19Ba-RAE.CEL 3.45 Mb
NINDS-CON-DG-19Ba-RAE.CHP 3.43 Mb
NINDS-CON-DG-22Ba-RAE.CEL 3.45 Mb
NINDS-CON-DG-22Ba-RAE.CHP 3.43 Mb
NINDS-CON-DG-26Ba-RAE.CEL 3.45 Mb
NINDS-CON-DG-26Ba-RAE.CHP 3.43 Mb
NINDS-CON-DG-3Ba-RAE.CEL 3.45 Mb
NINDS-CON-DG-3Ba-RAE.CHP 3.43 Mb
NINDS-CON-DG-A5a-RAE.CEL 3.45 Mb
NINDS-CON-DG-A5a-RAE.CHP 3.43 Mb
NINDS-CON-DG-A8a-RAE.CEL 3.45 Mb
NINDS-CON-DG-A8a-RAE.CHP 3.43 Mb
NINDS-PR-CA1-11a-RAE.CEL 3.45 Mb
NINDS-PR-CA1-11a-RAE.CHP 3.43 Mb
NINDS-PR-CA1-11a-RAE.DAT 40.16 Mb
NINDS-PR-CA1-15a-RAE.CEL 3.45 Mb
NINDS-PR-CA1-15a-RAE.CHP 3.43 Mb
NINDS-PR-CA1-1Ba-RAE.CEL 3.45 Mb
NINDS-PR-CA1-1Ba-RAE.CHP 3.43 Mb
NINDS-PR-CA1-4a-RAE.CEL 3.45 Mb
NINDS-PR-CA1-4a-RAE.CHP 3.43 Mb
NINDS-PR-CA1-8a-RAE.CEL 3.45 Mb
NINDS-PR-CA1-8a-RAE.CHP 3.43 Mb
NINDS-PR-CA1-9a-RAE.CEL 3.45 Mb
NINDS-PR-CA1-9a-RAE.CHP 3.43 Mb
NINDS-PR-CA1-A25a-RAE.CEL 3.45 Mb
NINDS-PR-CA1-A25a-RAE.CHP 3.43 Mb
NINDS-PR-CA1-A3a-RAE.CEL 3.45 Mb
NINDS-PR-CA1-A3a-RAE.CHP 3.43 Mb
NINDS-PR-CA3-11a-RAE.CEL 3.45 Mb
NINDS-PR-CA3-11a-RAE.CHP 3.43 Mb
NINDS-PR-CA3-15a-RAE.CEL 8.40 Mb
NINDS-PR-CA3-15a-RAE.CHP 202.99 Kb
NINDS-PR-CA3-1a-RAE.CEL 3.45 Mb
NINDS-PR-CA3-1a-RAE.CHP 3.43 Mb
NINDS-PR-CA3-4a-RAE.CEL 3.45 Mb
NINDS-PR-CA3-4a-RAE.CHP 3.43 Mb
NINDS-PR-CA3-8a-RAE.CEL 3.45 Mb
NINDS-PR-CA3-8a-RAE.CHP 3.43 Mb
NINDS-PR-CA3-9a-RAE.CEL 3.45 Mb
NINDS-PR-CA3-9a-RAE.CHP 3.43 Mb
NINDS-PR-CA3-A25a-RAE.CEL 3.45 Mb
NINDS-PR-CA3-A25a-RAE.CHP 3.43 Mb
NINDS-PR-CA3-A3a-RAE.CEL 3.45 Mb
NINDS-PR-CA3-A3a-RAE.CHP 3.43 Mb
NINDS-PR-DG-11a-RAE.CEL 3.45 Mb
NINDS-PR-DG-11a-RAE.CHP 3.43 Mb
NINDS-PR-DG-15Ba-RAE.CEL 3.45 Mb
NINDS-PR-DG-15Ba-RAE.CHP 3.43 Mb
NINDS-PR-DG-1Ba-RAE.CEL 3.45 Mb
NINDS-PR-DG-1Ba-RAE.CHP 3.43 Mb
NINDS-PR-DG-4Ba-RAE.CEL 3.45 Mb
NINDS-PR-DG-4Ba-RAE.CHP 3.43 Mb
NINDS-PR-DG-8a-RAE.CEL 3.45 Mb
NINDS-PR-DG-8a-RAE.CHP 3.43 Mb
NINDS-PR-DG-9a-RAE.CEL 3.45 Mb
NINDS-PR-DG-9a-RAE.CHP 3.43 Mb
NINDS-PR-DG-A25Ba-RAE.CEL 3.45 Mb
NINDS-PR-DG-A25Ba-RAE.CHP 3.43 Mb
NINDS-PR-DG-A3a-RAE.CEL 3.45 Mb
NINDS-PR-DG-A3a-RAE.CHP 3.43 Mb
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