| Proposal
Detail |
| Grant: |
DC000217
|
| Status: |
Public
|
| Service Type: |
Start to Finish Profiling
|
| Study
Relevance: |
| The study is relevant to an understanding of the forces that lead to sex differences in the brain. Many neural and psychiatric diseases affect men and women differently, so the understanding of sex differences in brain function impacts on our understanding of why the male and female brain differ in their susceptibility to disease. |
| Hypothesis: |
| Gene expression differs in the male and female embryonic chicken brain. |
| Specific
Aim: |
| Using Affymetrix chicken arrays, we will measure the gene expression in male and female embryonic chicken brain. |
| Experimental
Procedure and Design: |
| 20 male and 20 female chicken embryos will serve as source of brain tissue. In late stage embryos, we will remove the brain tissue and extract total RNA. Four birds will comprise each individual sample. Thus, we will have 5 biologically independent male samples, and an equal number of female samples. |
| Quality Control Description: |
| Each RNA sample will be a pool of RNA from 4 individual animals. Pooling will reduce inter-sample variability and allow better detection of sex differences.
We will use 5 statistically independent samples, which will allow an assessment of inter-sample variability which will form the basis for assessing the significance of inter-group differences. |
| Quality Control Types: |
|
biological_replicate
|
| Replicate Description: |
| Each RNA sample will be a pool of RNA from 4 individual animals. Pooling will reduce inter-sample variability and allow better detection of sex differences.
We will use 5 statistically independent samples, which will allow an assessment of inter-sample variability which will form the basis for assessing the significance of inter-group differences. |
| Replicate Types: |
|
biological_replicate
|
| Experimental Factors: |
|
Conditions that are tested in the experiment. At least one is required. Experimental factors are the independent variables in the experiment.
|
|
|
Factor Name
|
Description
|
Factor Category
|
|
sex
|
WE will compare males and females
|
sex
|
|
|
