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View Project Koh-7K08NS002068-05
Project Summary
Status: Public  
Publications: 1 Published
 		 
Project Detail Data Detail
Platform: Affymetrix MIAME Areas Compliance
Species: Rat Array Design Detail true
Organ/Tissue Type: brain Experiment Detail false
Organ Region: hippocampus Sample Detail false
Cell Type: HEK293 cells and CB1HEK293 cells Hybridization Detail false
Study Type: pharmacogenomic Measurement Detail false
Disease/Condition: 21q monosomy
Replicates: 3
Expected Samples: 12 
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Available Actions
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Investigator Contact Detail
Name Sookyong Koh
Street Address: BOX 51 Division of Neurology, Children's Memorial Hospital
Northwestern Med School, 2300 Children's Plaza
City, State/Province: Chicago , IL
Zip/Postal Code: 60614
Country: United States
Work Phone: 773-880-3179
Fax: 773-880-3374
E-mail: skoh@childrensmemorial.org
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Proposal Detail
Grant: 7K08NS002068-05
Status: Public
Service Type: Start to Finish Profiling
IACUC: 2003-11
IACUC date: 1998-09-30
Study Relevance:
Neuropsychiatric consequences of poorly controlled seizures that begin in childhood can be devastating. School failure or behavioral difficulty in a child with epilepsy is common and can become the focus of concern for families. Current antiepileptic drugs compound problems with their CNS side effects; effective therapy is currently limited as little is known about the cellular and molecular changes caused by seizures in the developing brain. This study will investigate transcriptional regulation induced by early-life seizures and explore alternative nonpharmacological therapeutic strategies in reversing damages of early-life seizures. We will study the therapeutic efficacy of environmental enrichment in reducing seizure-induced neuronal injury and in modifying gene expression alterations. We will explore molecular mechanisms underlying the beneficial effects of enriched environment and examine how different genes act in concert to influence the outcome of seizure-induced damage.
Hypothesis:
We hypothesize that environmental enrichment enhances plastic, homeostatic response of immature brain to excessive, dysregulating seizure activity and protect against maladaptive response of developing animals to seizures.
Specific Aim:
To test the effect of environmental enrichment in modifying KA seizure-induced alterations in gene expression.
Experimental Procedure and Design:
After inducing seizures by systemic injection of KA (8 mg/kg) or PBS injections (control) at P20, we will randomly assign P21 male Long Evans rats to 4 groups: enrichment housing (control-enriched; SE-enriched) or to standard vivarium cages (control; SE). Sixteen animals (8 control-enriched; 8 SE-enriched) will be housed as a group in a plastic rectangular cage measuring 115cm x 80 cm containing a running wheel, tunnels, rubber balls, a maze, a mirror, and a clock with a cyclist pendulum. Control and KA will be housed individually in a standard cage. Four litters will be used for each run of experiment and the experiment will be repeated once to obtain 16 animals per group. At P30, 240 h after KA or PBS, animals will be deeply anesthetized with isoflurane, decapitated for total RNA preparation (half brain for each, n = 12 per group). Total RNA will be isolated from each hippocampus individually, and equal amounts of RNA from 4 hippocampi will be pooled for each Genechip. Three independent hybridizations will be performed per condition (total of 12 Genechip Rat Expression Set 230).
Experimental Factors:
Conditions that are tested in the experiment. At least one is required. Experimental factors are the independent variables in the experiment.
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Experimental Factors is empty.
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Project Samples  This section lists the samples that are associated with this project. Individual sample details can be viewed by clicking on the View Sample icon to the right of the sample. If samples are selectable for analysis or for addition to a virtual
Samples associated with this project.
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Name Description Bio-Source Extracts  
C1 rat hippocampi (pooled from... BioSource12 1
C2 rat hippocampi (pooled from... BioSource13 1
C3 rat hippocampi (pooled from... BioSource14 1
KA1 rat hippocampi (pooled from... BioSource15 1
KA2 rat hippocampi (pooled from... BioSource16 1
KA3 rat hippocampi (pooled from... BioSource17 1
ENC1 rat hippocampi (pooled from... BioSource18 1
ENC2 rat hippocampi (pooled from... BioSource19 1
ENC3 rat hippocampi (pooled from... BioSource20 1
ENKA1 rat hippocampi (pooled from... BioSource21 1
ENKA2 rat hippocampi (pooled from... BioSource22 1
ENKA3 rat hippocampi (pooled from... BioSource23 1
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Project Hybridizations 

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View Hybridization View Hybridization  
Name Array Labeled Extract Hybridization Protocol  
RAE230A_1_hyb RAE230A_1 C1_e1_le1
RAE230A_2_hyb RAE230A_2 C2_e1_le1
RAE230A_3_hyb RAE230A_3 C3_e1_le1
RAE230A_4_hyb RAE230A_4 KA1_e1_le1
RAE230A_5_hyb RAE230A_5 KA2_e1_le1
RAE230A_6_hyb RAE230A_6 KA3_e1_le1
RAE230A_7_hyb RAE230A_7 ENC1_e1_le1
RAE230A_8_hyb RAE230A_8 ENC2_e1_le1
RAE230A_9_hyb RAE230A_9 ENC3_e1_le1
RAE230A_10_hyb RAE230A_10 ENKA1_e1_le1
RAE230A_11_hyb RAE230A_11 ENKA2_e1_le1
RAE230A_12_hyb RAE230A_12 ENKA3_e1_le1
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Project Reference Files
File Name Size
Signal Values for all 30 Samples.TXT 2.09 Mb
Signal Values for all 30 Samples.xls 9.21 Mb
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