spacer image

View Project sabba-affy-rat-168529
Project Summary
Status: Public  
Publications: 1 Published
1 In Preparation
 		 
Project Detail Data Detail
Platform: Affymetrix MIAME Areas Compliance
Species: Rat Array Design Detail true
Organ/Tissue Type: Multiple Organs Experiment Detail true
Organ Region: adrenal medulla Sample Detail false
Cell Type: mixed cell types Hybridization Detail false
Study Type: pharmacogenomic Measurement Detail false
Disease/Condition: normal
Replicates: 2
Expected Samples:  
spacer image
Available Actions
spacer image
Investigator Contact Detail
Name Esther Sabban
Street Address: New York Medical College, Dept. Biochemistry & Mol. Biology
Basic Sciences Building
City, State/Province: Vahalla , NY
Zip/Postal Code: 10595
Country: United States
Work Phone: 914-594-4068
Fax: 914-594-4058
E-mail: sabban@nymc.edu
spacer image
Proposal Detail
Grant: NS28869
Status: Public
Service Type: Start to Finish Profiling
IACUC: 76-12-0504
IACUC date: 0004-05-25
Study Relevance:
Stress is a major risk factor for many cardiovascular and neuropsyhological disorders, and involved changes in gene expression
Hypothesis:
Stress triggers alterations in gene expresssion of many proteins, especially transcription factors. The target genes of repeated stress partially overlap, but differ from those altered by single stress.
Specific Aim:
Determine target genes affected by single or repeated immboilization stress.
Experimental Procedure and Design:
Sprague Dawley male rats were exposed to single 2 hr immobilization stress or 2 hrs immbolization stress each for six consecultive days. Controls for single stress were untreated, and controls for repeated stress were handled for six days.
Experimental Factors:
Conditions that are tested in the experiment. At least one is required. Experimental factors are the independent variables in the experiment.
spacer image
Factor Name Description Factor Category
stress Immobilization behavioral_stimulus
spacer image
Project Samples  This section lists the samples that are associated with this project. Individual sample details can be viewed by clicking on the View Sample icon to the right of the sample. If samples are selectable for analysis or for addition to a virtual
Samples associated with this project.
spacer image

Action Button Key
View Sample View Sample  
Name Description Extracts  
AC 1 Unstressed animals 1
AC 2 Unstressed animals 1
1XIMO 1 Rats were immobilized 2 hours 1
1XIMO 2 Rats were immobilized for 2... 1
6XIMO 1 Rats were immobilized 2hour... 1
6XIMO 2 Rats were immobilized 2 hou... 1
HC 1 Rats were handled daily for... 1
HC 2 Rats were handled daily for... 1
spacer image
Project Hybridizations 

Action Button Key
View Hybridization View Hybridization  
Name Array Labeled Extract Hybridization Protocol  
Control #1_1_hyb Control #1_1 Sample 1_le1
Control #1_2_hyb Control #1_2 Sample 2_le1
Control #1_3_hyb Control #1_3 Sample 3_le1
Control #1_4_hyb Control #1_4 Sample 4_le1
spacer image
Grey dot spacer image