View Project Sabban-5R01NS028869-11

Project Summary
Status:	Public
Publications:	1 Published
Project Detail		Data Detail
Platform:	Affymetrix	MIAME Areas	Compliance
Species:	Rat	Array Design Detail	true
Organ/Tissue Type:	brain	Experiment Detail	false
Organ Region:	adrenal medulla	Sample Detail	false
Cell Type:	cortical astrocytes	Hybridization Detail	true
Study Type:	pharmacogenomic	Measurement Detail	true
Disease/Condition:	Anesthetics
Replicates:	0
Expected Samples:

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Investigator Contact Detail
Name	Esther Sabban
Street Address:	New York Medical College, Dept. Biochemistry & Mol. Biology
	Basic Sciences Building
City, State/Province:	Vahalla , NY
Zip/Postal Code:	10595
Country:	United States
Work Phone:	914-594-4068
Fax:	914-594-4058
E-mail:	sabban@nymc.edu

Proposal Detail

Grant:

5R01NS028869-11

Status:

Public

Service Type:

Start to Finish Profiling

IACUC:

38-1-0403 & 28-1-0303

IACUC date:

2003-04-23

Study Relevance:

Stress leads to important adaptive physiological responses including release of catecholamines from the adrenal medulla. Repeated or prolonged stress increases the propensity for many disorders including cardiovascular disease and neuropsychiatric disorders. The susceptibility to stress is determined by a number of factors including prior experience with stress. It has been found that while there is adaptation to prolonged cold stress; it leads to sensitization when cold stressed animals are exposed to a different (novel) stressor. Our study aims to determine the mechanism of this adaptation and sensitization.

Hypothesis:

We hypothesize that during the course of cold stress there are alterations in gene expression of several neurotransmitter related genes and transcription factors. In cold adapted animals, there is increased susceptibility to stress due to sustained changes in expression of several transcription factors, such as FRA-2 which lead to an exaggerated response to the novel stressor.

Specific Aim:

We will determine the gene expression patterns in rat adrenal medulla in response to several times of cold stress. We will also determine the changes in gene expression with single immobilization stress in adrenal medulla of rat with and without preexposure to cold stress to investigate the mechanism of sensitization in cold adapted animals.

Experimental Procedure and Design:

Rats were exposed to cold stress (4oC) for 1, 7 or 28 days. A parallel group was kept at room temperature (unstressed). Unstressed or rats pre-exposed to 28 days cold were immobilized for 2 hrs and killed by decapitation. The adrenal medullae, separately from right and left side, have been dissected and individually frozen at -70oC. Our recent immunoblots with the tissue from one side revealed that there is increase in tyrosine hydroxylase and c-fos with the short term cold stress and in dopamine beta hydroxylase and Fra-2 even with the prolonged cold stress. The response of several transcription factors to immobilization stress was enhanced in the cold adapted animals. RNA will be prepared with RNAqueous-Micro Isolation kit from Ambion (alternatively we can ship the tissues). We will pool samples from 4 rats per treatment to control for variability.

Experimental Factors:

Conditions that are tested in the experiment. At least one is required. Experimental factors are the independent variables in the experiment.


Experimental Factors is empty.

Project Samples

Samples associated with this project.

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Name	Description	Extracts
room temp - no stress	adrenal medulla	1
room temp -IMO	adrenal medulla 2 hr imo	1
1 day cold	Adreanl medulla 1 day cold ...	1
7 day cold	adrenal medulla 7 day cold	1
28 day cold stress	adrenal medualla 28 day cold	1
28 day cold plus IMO	adrenal medulla 28 day cold...	1

Project Hybridizations

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Name	Array	Labeled Extract	Hybridization Protocol
Hybridization6	Control #1_1	room temp - no stress _e1_le1	Affymetrix
Hybridization7	Control #1_2	room temp -IMO_e1_le1	Affymetrix
Hybridization8	Control #1_3	1 day cold_e1_le1	Affymetrix
Hybridization9	Control #1_4	7 day cold_e1_le1	Affymetrix
Hybridization10	Control #1_5	28 day cold stress _e1_le1	Affymetrix
Hybridization11	Control #1_6	28 day cold plus IMO_e1_le1	Affymetrix

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