View Project Hussaini-2R01NS035122-06A1

Project Summary
Status:	Public
Publications:	2 Published
Project Detail		Data Detail
Platform:	Affymetrix	MIAME Areas	Compliance
Species:	Human	Array Design Detail	true
Organ/Tissue Type:	brain	Experiment Detail	false
Organ Region:	amygdala	Sample Detail	false
Cell Type:	cortical astrocytes	Hybridization Detail	true
Study Type:	subclassification	Measurement Detail	false
Disease/Condition:	Pituitary Adenomas
Replicates:	0
Expected Samples:

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Investigator Contact Detail
Name	Isa M Hussaini
Street Address:	Department of Pathology
	University of Virginia, Box 800904, MR5
City, State/Province:	CHARLOTTESVILLE , VA
Zip/Postal Code:	22908
Country:	United States
Work Phone:	434-924-0110
Fax:	434-924-9177
E-mail:	imh5c@virginia.edu

Proposal Detail

Grant:

2R01NS035122-06A1

Status:

Public

Service Type:

Start to Finish Profiling

IRB:

HIC #10702

IRB date:

2003-05-16

Study Relevance:

Pituitary adenomas are common benign neoplasms giving rise to disorders of growth, reproductive function and cortisol production. Although recently determined to be monoclonal, very little is known about the mechanisms regulating the development of pituitary hyperplasia and neoplasia in humans. Surgical resection is the treatment of choice for most symptomatic pituitary adenomas. The goal of surgery is the complete removal of the tumor and the success of surgery is strongly affected by the presence of local invasion. However, complete tumor removal is unlikely when there is extensive local invasion. Identifying genes that control the invasiveness and recurrence of this class of tumors will provide therapeutic targets for this class of tumors.

Hypothesis:

We hypothesize that the differential expression and activation of a number of genes affect pituitary adenoma recurrence and invasiveness.

Specific Aim:

We will determine the expression pattern of genes in recurrent and invasive and pituitary adenomas and compare those to non-invasive and non-recurrent tumors.

Experimental Procedure and Design:

Rationale: Preliminary result showed a differential expression of novel PKC isozymes in non-invasive and invasive pituitary adenomas. PMA, an activator of both novel and classical PKC isozymes increased the expression of gelatinase A (MMP-2) mRNA in human pituitary adenoma cell line. This result raises a fundamental question as to the functional role of novel PKC isozymes and proteases in the invasive phenotype of pituitary adenomas. The primary component of this Specific Aim is to determine whether specific genes are differentially expressed in recurrent or invasive adenomas when compared with control non-invasive tumors. Tissue specimens from non-invasive and invasive (dural invasion based microscopic examination) and recurrent vs non-recurrent tumors will be used for microarray analysis. Frozen pituitaryspecimens will be collected and total RNA extracted with TriZol reagent. We will provide total RNA (10 ug) from non-invasive, invasive, reoccurred and non-occurrent pituitary adenomas.

Experimental Factors:

Conditions that are tested in the experiment. At least one is required. Experimental factors are the independent variables in the experiment.


Experimental Factors is empty.

Project Samples

Samples associated with this project.

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Name	Description	Bio-Source	Extracts
Control 1	RESECTED HUMAN PITUITARY TU...	29813	1
Control 2	RESECTED HUMAN PITUITARY TU...	29814	1
Control 3	RESECTED HUMAN PITUITARY TU...	29815	1
Control 4	RESECTED HUMAN PITUITARY TU...	29816	1
Control 5	RESECTED HUMAN PITUITARY TU...	29817	1
Experimental 1	RESECTED HUMAN PITUITARY TU...	29817	1
Experimental 2	RESECTED HUMAN PITUITARY TU...	29817	1
Control 1.1	HP75- Tumor Cell Line	29817	1
Experimental 1.1	HP75- Tumor Cell Line	29817	1
Sample 10	RESECTED HUMAN PITUITARY TU...	29817	1

Project Hybridizations

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Name	Array	Labeled Extract	Hybridization Protocol
Hybridization1	Human Genome U133 Plus 2.0 Array_1	Recurrent Pituitary Adenoma_e1_le1	Affymetrix
Hybridization2	Human Genome U133 Plus 2.0 Array_2	Non-recurrent pituitary adenoma_e1_le1	Affymetrix
Hybridization3	Human Genome U133 Plus 2.0 Array_3	Invasive pituitary adenoma_e1_le1	Affymetrix
Hybridization4	Human Genome U133 Plus 2.0 Array_4	Non-invasive pituitary adenoma_e1_le1	Affymetrix
Hybridization5	Human Genome U133 Plus 2.0 Array_5	Sample5_e1_le1	Affymetrix
Hybridization6	Human Genome U133 Plus 2.0 Array_6	Sample6_e1_le1	Affymetrix
Hybridization7	Human Genome U133 Plus 2.0 Array_7	Sample7_e1_le1	Affymetrix
Hybridization8	Human Genome U133 Plus 2.0 Array_8	Sample8_e1_le1	Affymetrix
Hybridization9	Human Genome U133 Plus 2.0 Array_9	Sample9_e1_le1	Affymetrix
Hybridization10	Human Genome U133 Plus 2.0 Array_10	Sample10_e1_le1	Affymetrix

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