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View Project Russell-3U24NS043571-01S1-2
Project Summary
Status: Public  
Publications: 1 Published
 		 
Project Detail Data Detail
Platform: Affymetrix MIAME Areas Compliance
Species: Human Array Design Detail true
Organ/Tissue Type: Experiment Detail false
Organ Region: Sample Detail false
Cell Type: fibroblast Hybridization Detail false
Study Type: pharmacogenomic Measurement Detail false
Disease/Condition: Active tumor
Replicates: 2
Expected Samples: 6 
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Available Actions
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Investigator Contact Detail
Name Paul Russell
Street Address: School of Veterinary Medicine, Unversity of Wisconsin
2015 Linden Drive
City, State/Province: Madison , WI
Zip/Postal Code: 35706
Country: United States
Work Phone: (608) 262-5652
Fax:
E-mail: russellp@svm.vetmed.wisc.edu
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Proposal Detail
Grant: 3U24NS043571-01S1
Status: Public
Service Type: Start to Finish Profiling
IRB: TBA
IRB date: 2003-09-03
Study Relevance:
TGF-beta levels are known to increase in the aqueous humor of eye cells in patients with glaucoma. Increase TGF-beta is assumed to have a biochemical impact on the trabecular meshwork, and an increase in extracellular matrix formation, which may be responsible for decrease outflow facility of the eye. This may increase extracellular pressure, causing glaucoma. TGF-beta 1 may be the cause of abnormal accumulation of extracellular matrices in trabecular meshwork of eyes with primary open angle glaucoma. Transforming growth factor (TGF)-beta2 regulates the expression of proteoglycans in aqueous humor from human glaucomatous eyes.
Hypothesis:
We expect to see a change in expression of the proteoglycans in HTM cells as a response to TGF-beta treatment.
Specific Aim:
To identify gene expression changes as a result of TGF-beta1 and 2 treatment of human trabecular meshwork cells.
Experimental Procedure and Design:
Human Trabecular Meswork cells in the eye were bathed by aqueous humor. TM cells were removed from individuals with the following ages: 16,66,67,73, and 76. Each individual was treated with EtOH (control), TGF-beta1, or TGF-beta2. Total RNA from each individual was pooled for each chip. Technical replicates were created for each treatment type, for a total of 6 chips.
Experimental Factors:
Conditions that are tested in the experiment. At least one is required. Experimental factors are the independent variables in the experiment.
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Experimental Factors is empty.
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Project Samples  This section lists the samples that are associated with this project. Individual sample details can be viewed by clicking on the View Sample icon to the right of the sample. If samples are selectable for analysis or for addition to a virtual
Samples associated with this project.
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Action Button Key
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Name Description Bio-Source Extracts  
Ctr TM 2a Human Trabecular Meshwork BioSource6 1
Ctr TM 2b Human Trabecular Meshwork BioSource7 1
TGF-beta1 TM 1a Human Trabecular Meshwork BioSource8 1
TGF-beta1 TM 1b Human Trabecular Meshwork BioSource9 1
TGF-beta2 TM 1a Human Trabecular Meshwork BioSource10 1
TGFbeta-2 TM 1b Human Trabecular Meshwork BioSource11 1
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Project Hybridizations 

Action Button Key
View Hybridization View Hybridization  
Name Array Labeled Extract Hybridization Protocol  
HG-U133A_1_hyb HG-U133A_1 Ctr TM 2a_e1_le1
HG-U133A_2_hyb HG-U133A_2 Ctr TM 2b_e1_le1
HG-U133A_3_hyb HG-U133A_3 TGF-beta1 TM 1a_e1_le1
HG-U133A_4_hyb HG-U133A_4 TGF-beta1 TM 1b_e1_le1
HG-U133A_5_hyb HG-U133A_5 TGF-beta2 TM 1a_e1_le1
HG-U133A_6_hyb HG-U133A_6 TGFbeta-2 TM 1b_e1_le1
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Project Reference Files
File Name Size
PHG-CtrTM-2aUA-s2.CEL 11.68 Mb
PHG-CtrTM-2bUA-s2.CEL 11.59 Mb
PHG-TGFbeta1TM-1aUA-s2.CEL 11.57 Mb
PHG-TGFbeta1TM-1bUA-s2.CEL 11.66 Mb
PHG-TGFbeta2TM-1aUA-s2.CEL 11.59 Mb
PHG-TGFbeta2TM-1bUA-s2.CEL 11.54 Mb
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